Chemical Sciences Molecular Absorption Spectroscopy Lab Experiments

Determination of unknown concentration of an analyte by using the Beer-Lambert Law

We know that the spectrophotometer is an instrument that separates electromagnetic radiation into its component wavelengths and selectively measures the intensity of radiation after passing through a sample. The quantitative measurement of light absorption as a function of wavelength can not only establish the identity but can also determine the concentration of an absorbing substance in solution. From the Beer–Lambert law we know that more concentrated solution absorbs more of the light.