Effects of sample concentration Dependence of Absorbance (Beer Law)
Pre-Assignments
- If all the light passes through a solution without any absorption, then what are the values of absorbance and percent transmittance?
- If all the light is absorbed, then what are the values of absorbance and percent transmittance?
- If A = 2.00, then what are the % of the photons absorbed and reach the detector?
- "The optical absorbance of a chromophore in a transparent solvent varies linearly with both the sample path length and the chromophore concentration." Is the statement true or false?
- What is the absorbance value when the absorption cross-section of a molecule is zero?
Post-Assignments
- "A dilute solution of a chromophore can absorb more light than that its concentrated solution." Justify the statement.
- Is the absorbance vs. concentration plot linear for any concentration range of a substance?
- Which data appears convenient to handle for quantitative analysis - absorbance data or transmittance data- and why?
- What is the necessity of constructing more than one plot of absorbance vs. concentration for more than one wavelength?